Sky Journal of Microbiology Research Vol. 5(2), pp. 010-017, March, 2017. Available online, http://www.skyjournals.org/SJMR
ISSN 2315-876X ©2017 Sky Journals

 

Full Length Research Paper

Bioconversion of agricultural cellulose waste to valuable product by cellulolytic nitrogen-fixing bacterial isolates

San San Yu1, Zaw Ko Latt2, Ei Phyu Kyaw2 and Tin Mar Lynn2

 

1Microbiology Laboratory, Biotechnology Research Department, Kyaukse, Myanmar, 95-92401379.
2Microbiology Laboratory, Biotechnology Research Department, Kyaukse, Myanmar.
 

*Corresponding author. E-mail: sakurasan2007@gmail.com

 

Accepted 8 April, 2017

 

Abstract

Six strains of cellulolytic nitrogen-fixing bacteria were isolated from various agricultural soil sources of Kyaukse District, Mandalay Division, Myanmar by using nitrogen free Cellulose and Carboxymethyl Cellulose (CMC) medium. From the 16S rDNA sequencing results, three strains showed high similarity to Lysobacter enzymogenes and three strains showed high similarity to Azotobacter chroococcum. These six isolates excreted significant amounts of ammonium (30.03 – 100.32 ppm). Cellulolytic activity of isolated strains was detected by DNS colorimetric method using CMC and cellulose as substracts and significant amount of glucose was observed. Phosphate solubilizing, IAA (Indole Acetic Acid) production activity and antifungal activities of isolated strains were also detected. The phosphate solubilizing amount of these isolates was detected form 11 to 26 ppm. Among six strains, four bacterial isolates can also produce IAA. These strains gave positive results to Indole test. Antifungal activity of isolated strains was tested with Fusarium, Rhizotonia and Pythirium. M-2 and M-3 isolates can inhibit the growth of Fusarium and Rhizotonia. M-1 isolate can inhibit the growth of Fusarium, Ey-1 can inhibit Rhizotonia and E-4 can inhibit Pythirium. Composting was studied by using 6 strains on straw waste and complete composting was detected for a month. Formulated Biofertilizer based on compost by adding 7 strains including Sachromycese cerevisiae was applied on rice cultivation and good yield was obtained.

 

Key words: Cellulolytic nitrogen-fixing bacteria, Lysobacter enzymogenes, Azotobacter chroococcum composting, biofertilizer, rice cultivation.

 

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