Sky Journal of Microbiology Research Vol. 4(4), pp. 021-030, June, 2016. Available online,
ISSN 2315-876X ©2016 Sky Journals


Full Length Research Paper

Comparative bioremediation of crude oil – contaminated soil samples using activated soil and activated cow dung

Ikuesan, Felix Adeleke*, Boboye, Bolatito Esther and Adetuyi, Fatusa Clement


Department of Microbiology, The Federal University of Technology, P. M. B., 704, Akure, Ondo State, Nigeria.


*Corresponding author. E-mail: Tel.: +2348033842588.    


Accepted 27 May, 2016



This research studied the relative potentials of activated soil and activated cow dung in bioremediation of crude oil contaminated soils. Crude oil degrading microorganisms were isolated and identified from soil and cow dung samples before activation. Activated soil and activated cow dung prepared with 1% (w/w) crude oil were evaluated for their potentials in bioremediation of three agricultural soil samples of Igodan - Lisa, Oba-Ile and Idoani in Ondo State, Nigeria. The activated materials were applied at 5 and 10% (w/w) rates to 10% (w/w) experimentally crude oil contaminated soil samples. Crude oil loss in samples was estimated fortnightly for 16 weeks by the weight loss method using n-hexane as extractant. Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Klebsiella edwardsii, Escherichia coli, Staphylococcus hominis, Actinomyces bovis, Trichoderma species, Kodamaea ohmeri, Mucor mucedo, Aspergillus species, Scopulariopsis brevicaulis, Fusarium species were among the crude oil degrading microorganisms obtained from the samples. The activated cow dung exhibited higher percentage (52.12 – 60.23%) of crude oil loss than activated soil (39.84 - 44.16%) at both 5 and 10% levels of activated materials addition. The results of this study suggest the relative potentials of activated cow dung in the enhancement of bioremediation of crude oil polluted agricultural soils.


Key words: Crude oil, contaminated soil, bioremediation, activated cow dung enhancement, agricultural soils, Ondo State.


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