Sky Journal of Microbiology Research Vol. 4(5), pp. 042-046, July, 2016. Available online, http://www.skyjournals.org/SJMR
ISSN 2315-876X ©2016 Sky Journals

 

Full Length Research Paper

Antibiotic susceptibility profile of Staphylococcus aureus isolated from healthy chickens in poultry farms in Kano state, Nigeria

Bala H. K1*, Igwe J. C.2, Olayinka B. O., Olonitola O. S., Onaolapo J. A.1 and Cordelia N. Okafo3

 

1Department of Pharmaceutics and Pharmaceutical Microbiology, Ahmadu Bello University, Zaria.
2Department of Medical Biotechnology, National Biotechnology Development Agency, Abuja.
3National Water Resources Institute, Kaduna, Nigeria.

 

*Corresponding author. E-mail: igwejames42@yahoo.com.   

 

Accepted 27 June, 2016

 

Abstract

Staphylococcus aureus infection in poultry birds have been linked to the cause of increased morbidity and mortality in poultry management and treatment due to antibiotic resistance. This aim of this study is to proffer better treatment option in the management of S. aureus infection from poultry farms in Kano State using standard microbiological methods. The result showed that out of the 1260 samples collected from 12 farms in Kano State, 600 were from cloacae, 600 from nostril and 60 were from poultry farm workers. Using conventional identification methods, 562 isolates showed presumptive characteristics of S. aureus. The use of STAPH Agglutination kit reduced the number of the isolates to 290 while further identification method using Microgen STAPH kit confirmed 33.8% (98) of the presumptive isolates to be S. aureus. 8.3% (5) of the confirmed S. aureus were from the nostrils of poultry farm works. The antibiotic susceptibility profile study showed that high percentage (71.4%) of the isolates was resistant to Oxytetracycline, Oxacillin and Ampicillin, 61.2% to Chloramphenicol, 55% to Erythromycin and 30.6% to Cefoxitin. Vancomycin showed high activity against 74.5% of the isolates, Augumentin 69.4%, Ciprofloxacin 64.3% and Gentamicin 60.2%. Three of the isolates were observed to be resistant to all the 12 antibiotics tested while only one isolate was susceptible to all the antibiotics tested. Statistical analysis showed that isolates from layers were more resistant than those from broiler and poultry farm workers at p ≤ 0.05 level of significance. We conclude that the isolates from this study have varied antibiotic susceptibility pattern and that Vancomycin, Augumentin, Ciprofloxacin and Gentamicin could still be proficient in the management of S. aureus infection from poultry farm in Kano State, but surveillance and publicity should be carried out to curtail the wide spread of antibiotic resistance induced through irrational antibiotic misuse.

 

Key words: Poultry farm, antibiotics susceptibility profile, Staphylococcus aureus, resistance.

 

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