Sky Journal of Medicines and Medical Sciences Vol. 6(4), pp. 020-026, September, 2020, Available online, http://www.skyjournals.org/SJMMS

ISSN 2315-8808 ©2020 Sky Journals

 

Full Length Research Paper

Isolation and antifungal responses of Candida spp from Candidemia patient in Saudi Arabia
 

Abdullah Hani*, Kabila Salah and Qadri Ishtaiq

 

King Abdulaziz University, Saudi Arabia – Jeddah, Department of Biology, Division of Microbiology, Faculty of Sciences.
 

*Corresponding author. E-mail:  hannii4@hotmail.com.

 

Accepted  12 September, 2020

 

Abstract

 

Candidemia is an infection that demonstrates a significant morbidity and mortality risk and the most common type of nosocomial in a different country. In Saudi Arabia, the variable has been reported to be ranging from 3 to 42 episodes of candidemia per year. Candida albicans is the most prevalent species and they account for candidemia, followed by Candida parapsilosis, Candida tropicalis, Candida glabrata, Candida krusei. Studies of the sensitivity of Candida species to antifungal agents note that the minority of C. albicans isolates was resistant to fluconazole and unexpectedly high rate of resistance was observed in C. parapsilosis. A tremendous high rate of caspofungin resistance in C. glabrata and C. krusei was observed. Used three methods for determination species of candida, first one conventional by Chromogenic agars (CHRO Magar) second automation by Vitek-2 system (version 7.01, BioMérieux, Inc., Durham, USA) final were done using molecular identification. Compilation of positive candida specie Using three different methods to identify the species of the candida, were matched Candida approximately 81.8%. The C. parapsilosis was the highest isolate among other species. The sensitivity test by five types of antifungals with a Vitek-2. Two strains of C. krusei were resistant to Fluconazole (MIC 8) and Flucytosine (MIC 8), one strain of C. albicans appeared resistant to Voriconazole (MIC4), intermittently (MIC 32) result were for C. parapsilosis. Phylogenetic analyses to 22 isolate of Candida spp. compare each isolation with 2 of the isolates recorded in Genbank database. In the research upon identification, we do not rely on one type of identification method on the Candida. We need more than one method to obtain the correct definition.

Key words: Candida, antifungal, candidemia, isolation.

 

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