Sky Journal of Biochemistry Research Vol. 5(6), pp. 069-074, December, 2016.  Available online http://www.skyjournals.org/SJBR

ISSN 2315-8786 ©2016 Sky Journals

 

Full Length Research Paper

Antioxidant activity of aqueous extract of Spondias mombin (plum) leaf on haematological parameters in iron deficient rats
 

Abdulkarim M.*, Atiku M. K. and Bala M.

 

Department of Biochemistry, Bayero University Kano, P. M. B., 3011, Kano, Nigeria.  

 

Accepted 22 September, 2016

 

Abstract

 

Spondias mombin is relied on for various herbal remedies for numerous conditions. This study is to evaluate the antioxidant effect of mombin in iron deficient rats. The effects of oral administration of aqueous extract of Spondias mombin leaf at doses of 100, 200 and 300 mg/kg body weight on the organ-body weight ratio, lipid peroxidation and antioxidant enzyme (catalase and superoxide dismutase) activities of iron sufficient and iron deprived rats were investigated. Forty (40) albino rats (45 – 55 g) were used for the study. Ten (10) rats were fed with iron sufficient diet (control group) and thirty (30) rats were made iron deficient by feeding them with iron deprived diet. After four weeks of feeding the rats, their hematological parameters such as haemoglobin concentration (Hb), packed cell volume (PCV), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular volume (MCH) and mean corpuscular haemoglobin concentration (MCHC) were determined. The results (PCV, Hb, MCH) of the iron deprived rats were found to be significantly (p < 0.05), reduced when compared with rats fed on iron sufficient feed, an indication that the rats fed with iron deprived diet were anaemic. The anaemic rats were then treated with various doses of the extract for two weeks. Phytochemical screening of the extract revealed the presence of alkaloids, tannins, flavonoids, saponins and cardiac glycosides. There was a significant (p < 0.05) decrease in the liver (due to lack of iron affecting the biological processes in the liver), body weight ratios of the untreated iron deprived rats when compared with the control and other groups but the decrease was only significant in SM 100 group. There was also significant decrease (p < 0.05) in the catalase and superoxide dismutase activity of rats in the iron deprived group (IDG) when compared with other groups. There was a significant (p>0.05) increase in the level of malondialdehyde in liver of untreated iron deficient rats when compared with the control (iron sufficient group). The results obtained suggest that the administration of aqueous extract of Spondias mombin leaf might have restored the activity of antioxidant enzymes and decrease lipid peroxidation.

 

Key words: Antioxidant Spondias mombin, aneamia, lipid peroxidation.

 

 

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